2022

Jugder BE, Batista JH, Gibson JA, Cunningham PM, Asara JM, Watnick PI, Vibrio cholerae high cell density quorum sensing activates the host intestinal innate immune response, Cell Reports, 2022.

DOI:https://doi.org/10.1016/j.celrep.2022.111368

Chen Y, Rudolph SE, Longo BN, Pace F, Roh TT, Condruti R, Gee M, Watnick PI, Kaplan DL, Bioengineered 3D Tissue Model of Intestine Epithelium with Oxygen Gradients to Sustain Human Gut Microbiome. Advanced Healthcare Materials, 2022.

• DOI: 10.1002/adhm.202200447

Gibson JA, Gebhardt MJ, Santos ERS, Dove SL, Watnick PI, Sequestration of a dual function DNA-binding protein by Vibrio cholerae CRP, PNAS, 119 (46), 2022.

https://www.pnas.org/doi/abs/10.1073/pnas.2210115119

2021

Pace F, Rudolph SE, Chen Y, Bao B, Kaplan D, and Watnick PI, The short-chain fatty acids proionate and butyrate augment adherent-invasive Escherichia coli virulence but repress inflammation in a human intestinal enteroid model of infection. Microbiology Spectrum, 2021. https://doi.org/10.1128/Spectrum.01369-21

Jugder B-E, Kamareddine L, Watnick PI, Microbiota-derived acetate activates intestinal innate immunity via the Tip60 histone acetyltransferase complex, Immunity, 2021. https://doi.org/10.1016/j.immuni.2021.05.017

2020

Pace F, Watnick PI, The interplay of sex steroids, the immune response, and the intestinal microbiota, Trends in Microbiology, 2020. DOI: 10.1016/j.tim.2020.11.001

Vanhove AS, Jugder BE, Barraza D, Watnick PI, Identification of Vibrio cholerae methionine acquisition systems elucidates methionine availability in the arthropod intestine, Applied and Environmental Microbiology, 2020, in press.

Jugder BE, Watnick PI, Vibrio cholerae sheds its coat to make itself comfortable in the gut, Cell Host Microbe, 2020. doi: 10.1016/j.chom.2020.01.017

2019

Watnick PI, Jugder BE, Microbial control of intestinal homeostasis via enteroendocrine cell innate immune signaling, Trends in Microbiology, 2019. DOI:https://doi.org/10.1016/j.tim.2019.09.005

2018

Liao J, Xu G, Mevers EE, Clardy J, Watnick PI, A high-throughput, whole cell assay to identify compounds active against carbapenem-resistant Klebsiella pneumoniae, PLoS ONE, 2018. https://doi.org/10.1371/journal.pone.0209389.

Vijayakumar V, Vanhove AS, Pickering BS, Liao J, Tierney BT, Asara JM, Bronson R,  Watnick PI, Removal of a membrane anchor reveals the opposing regulatory functions of Vibrio cholerae glucose-specific Enzyme IIA in biofilms and the mammalian intestine, MBio, 2018. DOI: 10.1128/mBio.00858-18

Kamareddine L, Robins WP, Berkey CD, Mekalanos JJ, Watnick PI, The Drosophila Immune Deficiency Pathway modulates enteroendocrine function and host metabolism, Cell Metabolism, 2018, https://doi.org/10.1016/j.cmet.2018.05.026.

Liao J, Gibson JA, Pickering BS, and Watnick PI, Sublingual adjuvant delivery by a live attenuated Vibrio cholerae-based antigen presentation platform, mSphere. 2018 June; 3(3). pii: e00245-18. doi: 10.1128.

Liao J, Smith DR, Brynjarsdóttir J, Watnick PI, A self-assembling whole cell vaccine antigen presentation platform, J Bacteriol. 2018, Feb 26. pii: JB.00752-17. doi: 10.1128/JB.00752-17.

Kamareddine L, Wong ACN, Vanhove AS, Hang S, Purdy AE, Kierek-Pearson K, Asara JM, Ali A, Morris JG, Watnick PI, Activation of Vibrio cholerae quorum sensing promotes survival of an arthropod host. Nature Microbiol. 2018 Feb; 3(2): 243-25.

2017

Vanhove AS, Hang S, Vijayakumar V, Wong AC, Asara JM, Watnick PI. Vibrio cholerae ensures function of host proteins required for virulence through consumption of luminal methionine sulfoxide. PLoS Pathog. 2017 Jun 6;13(6):e1006428.

Alawdah LS, Campbell JN, Pollock N, Watnick PI. Erysipelothrix rhusiopathiae Suppurative Arthritis in a 12-year-old Boy After an Unusual Fresh Water Exposure. Pediatr Infect Dis J. 2017 Apr;36(4):431-433.

2016

Wong AC, Vanhove AS, Watnick PI. The interplay between intestinal bacteria and host metabolism in health and disease: lessons from Drosophila melanogaster. Dis Model Mech. 2016 Mar;9(3):271-81.

Leng Y, Vakulskas CA, Zere TR, Pickering BS, Watnick PI, Babitzke P, Romeo T. Regulation of CsrB/C sRNA decay by EIIA(Glc) of the phosphoenolpyruvate:carbohydrate phosphotransferase system. Mol Microbiol. 2016 Feb;99(4):627-39.

2015

Smith DR, Maestre-Reyna M, Lee G, Gerard H, Wang AH, Watnick PI. In situ proteolysis of the Vibrio cholerae matrix protein RbmA promotes biofilm recruitment. Proc Natl Acad Sci U S A. 2015 Aug 18;112(33):10491-6.

2014

Hang S, Purdy AE, Robins WP, Wang Z, Mandal M, Chang S, Mekalanos JJ, Watnick PI. The acetate switch of an intestinal pathogen disrupts host insulin signaling and lipid metabolism. Cell Host Microbe. 2014 Nov 12;16(5):592-604.

Pickering BS, Lopilato JE, Smith DR, Watnick PI. The transcription factor Mlc promotes Vibrio cholerae biofilm formation through repression of phosphotransferase system components. J Bacteriol. 2014 Jul;196(13):2423-30.

2013

Guichard A, Cruz-Moreno B, Aguilar B, van Sorge NM, Kuang J, Kurkciyan AA, Wang Z, Hang S, Pineton de Chambrun GP, McCole DF, Watnick P, Nizet V, Bier E. Cholera toxin disrupts barrier function by inhibiting exocyst-mediated trafficking of host proteins to intestinal cell junctions. Cell Host Microbe. 2013 Sep 11;14(3):294-305.

Wang Z, Hang S, Purdy AE, Watnick PI. Mutations in the IMD pathway and mustard counter Vibrio cholerae suppression of intestinal stem cell division in Drosophila. MBio. 2013 Jun 18;4(3):e00337-13.

Ymele-Leki P, Houot L, Watnick PI. Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation. Appl Environ Microbiol. 2013 Aug;79(15):4675-83.

2012

Pickering BS, Smith DR, Watnick PI. Glucose-specific enzyme IIA has unique binding partners in the Vibrio cholerae biofilm. MBio. 2012 Nov 6;3(6):e00228-12.

Absalon C, Ymele-Leki P, Watnick PI. The bacterial biofilm matrix as a platform for protein delivery. MBio. 2012 Jul 17;3(4):e00127-12.

Wang Z, Berkey CD, Watnick PI. The Drosophila protein mustard tailors the innate immune response activated by the immune deficiency pathway. J Immunol. 2012 Apr 15;188(8):3993-4000.

Ymele-Leki P, Cao S, Sharp J, Lambert KG, McAdam AJ, Husson RN, Tamayo G, Clardy J, Watnick PI. A high-throughput screen identifies a new natural product with broad-spectrum antibacterial activity. PLoS One. 2012;7(2):e31307.

2011

Purdy AE, Watnick PI. Spatially selective colonization of the arthropod intestine through activation of Vibrio cholerae biofilm formation. Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19737-42.

Absalon C, Van Dellen K, Watnick PI. A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. PLoS Pathog. 2011 Aug;7(8):e1002210.

2010

Houot L, Chang S, Pickering BS, Absalon C, Watnick PI. The phosphoenolpyruvate phosphotransferase system regulates Vibrio cholerae biofilm formation through multiple independent pathways. J Bacteriol. 2010, Jun;192(12):3055-67.

Houot L, Chang S, Absalon C, Watnick PI. Vibrio cholerae phosphoenolpyruvate phosphotransferase system control of carbohydrate transport, biofilm formation, and colonization of the germ-free mouse intestine. Infect Immun. 2010 Apr;78(4):1482-94.

2009

Karatan E, Watnick P. Signals, regulatory networks, and materials that build and break bacterial biofilms. Microbiol Mol Biol Rev. 2009 Jun;73(2):310-47.

Berkey CD, Blow N, Watnick PI. Genetic analysis of Drosophila melanogaster susceptibility to intestinal Vibrio cholerae infection. Cell Microbiol. 2009 Mar;11(3):461-74.

2008

Van Dellen KL, Houot L, Watnick PI. Genetic analysis of Vibrio cholerae monolayer formation reveals a key role for ∆Ψ in the transition to permanent attachment. J Bacteriol. 2008 Dec;190(24):8185-96.

Houot L, Watnick PI. A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm. J Bacteriol. 2008 Jan;190(1):311-20.

2006

Van Dellen KL, Watnick PI, The Vibrio cholerae biofilm: a target for novel therapies to prevent and treat cholera, Drug Discovery Today: Disease Mechanisms, 2006; 3: 261-265.

2005

Karatan E, Duncan TR, Watnick PI. NspS, a predicted polyamine sensor, mediates activation of Vibrio cholerae biofilm formation by norspermidine. J Bacteriol. 2005 Nov;187(21):7434-43.

Blow NS, Salomon RN, Garrity K, Reveillaud I, Kopin A, Jackson FR, Watnick PI, Vibrio cholerae infection of Drosophila melanogaster mimics the human disease cholera. PLoS Pathog. 2005 Sep;1(1):e8.

Moorthy S, Watnick PI. Identification of novel stage-specific genetic requirements through whole genome transcription profiling of Vibrio cholerae biofilm development. Mol Microbiol. 2005 Sep;57(6):1623-35.

Kapfhammer D, Karatan E, Pflughoeft KJ, Watnick PI. Role for glycine betaine transport in Vibrio cholerae osmoadaptation and biofilm formation within microbial communities. Appl Environ Microbiol. 2005 Jul;71(7):3840-7.

2004

Moorthy S, Watnick PI. Genetic evidence that the Vibrio cholerae monolayer is a distinct stage in biofilm development. Mol Microbiol. 2004 Apr;52(2):573-87.

2003

Kierek K, Watnick PI. The Vibrio cholerae O139 O-antigen polysaccharide is essential for Ca2+-dependent biofilm development in sea water. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14357-62.

Pflughoeft KJ, Kierek K, Watnick PI. Role of ectoine in Vibrio cholerae osmoadaptation. Appl Environ Microbiol. 2003 Oct;69(10):5919-27.

Kierek K, Watnick PI. Environmental determinants of Vibrio cholerae biofilm development. Appl Environ Microbiol. 2003 Sep;69(9):5079-88.

Bomchil N, Watnick P, Kolter R. Identification and characterization of a Vibrio cholerae gene, mbaA, involved in maintenance of biofilm architecture. J Bacteriol. 2003 Feb;185(4):1384-90.

2002

Haugo AJ, Watnick PI. Vibrio cholerae CytR is a repressor of biofilm development. Mol Microbiol. 2002 Jul;45(2):471-83.

Watnick PI. Paula I Watnick–elucidating the role of biofilms. Interview by Pam Das. Lancet Infect Dis. 2002 Mar;2(3):190-2. PubMed PMID: 11944189.

2001

Watnick PI, Lauriano CM, Klose KE, Croal L, Kolter R. The absence of a flagellum leads to altered colony morphology, biofilm development and virulence in Vibrio cholerae O139. Mol Microbiol. 2001 Jan;39(2):223-35.

2000

Watnick P, Kolter R. Biofilm, city of microbes. J Bacteriol. 2000 May;182(10):2675-9.

Butterton JR, Choi MH, Watnick PI, Carroll PA, Calderwood SB. Vibrio cholerae VibF is required for vibriobactin synthesis and is a member of the family of nonribosomal peptide synthetases. J Bacteriol. 2000 Mar;182(6):1731-8.

1999

Watnick PI, Kolter R. Steps in the development of a Vibrio cholerae El Tor biofilm. Mol Microbiol. 1999 Nov;34(3):586-95.

O’Toole GA, Pratt LA, Watnick PI, Newman DK, Weaver VB, Kolter R. Genetic approaches to study of biofilms. Methods Enzymol. 1999;310:91-109.

Watnick PI, Fullner KJ, Kolter R. A role for the mannose-sensitive hemagglutinin in biofilm formation by Vibrio cholerae El Tor. J Bacteriol. 1999 Jun;181(11):3606-9.

1998

Watnick PI, Butterton JR, Calderwood SB. The interaction of the Vibrio cholerae transcription factors, Fur and IrgB, with the overlapping promoters of two virulence genes, irgA and irgB. Gene. 1998 Mar 16;209(1-2):65-70.

1997

Watnick PI, Eto T, Takahashi H, Calderwood SB. Purification of Vibrio cholerae fur and estimation of its intracellular abundance by antibody sandwich enzyme-linked immunosorbent assay. J Bacteriol. 1997 Jan;179(1):243-7.

1990

Watnick PI, Chan SI, Dea P. Hydrophobic mismatch in gramicidin A’/lecithin systems. Biochemistry. 1990 Jul 3;29(26):6215-21.

Watnick PI, Dea P, Chan SI. Characterization of the transverse relaxation rates in lipid bilayers. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2082-6.

1987

Watnick PI, Dea P, Nayeem A, Chan SI. Cooperative lengths and elastic constants in lipid bilayers: The chlorophyll a/ dimyristoyllecithin system. J. Chem. Phys. 1987; 86: 5789-5800.

1983

Jelinski LW, Dumais JJ, Watnick PI, Engel A, Sefcik MD. NMR relaxation in poly(butylene terephthalate) and poly(butylene terephthalate)-containing segmented copolymers. Macromolecules. 1983; 16: 409-415

1982

Gierasch LM, Lacy JE, Thompson KF, Rockwell AL, Watnick PI. Conformations of model peptides in membrane-mimetic environments. Biophys J. 1982 Jan;37(1):275-84.

Dismukes CG, Ferris K, Watnick P. EPR spectroscopic evidence for a tetranuclear manganese cluster as the site for photosynthetic oxygen evolution. Photobiochem. Photobiophys. 1982; 3: 243-56.

Jelinski LW, Dumais JJ, Watnick PI, Bass SV, Shepard L. ¹³C-NMR of ABS in the solid state. Characterization and relaxation studies. J. Polymer Sci. 1982; 20: 3285-96.

Thesis

Watnick PI. Cooperative properties of lipid bilayers: Collective director fluctuations and the effects of hydrophobic mismatch in protein/lipid membrane systems. Pasadena (CA): California Institute of Technology; 1988.